Characterization of Intact Neo-Glycoproteins by Hydrophilic Interaction Liquid Chromatography
نویسندگان
چکیده
منابع مشابه
Characterization of intact neo-glycoproteins by hydrophilic interaction liquid chromatography.
In this study, an HPLC HILIC-UV method was developed for the analysis of intact neo-glycoproteins. During method development the experimental conditions evaluated involved different HILIC columns (TSKgel Amide-80 and ZIC-pHILIC), and water-acetonitrile mixtures containing various types of acids and salts. The final selected method was based on a TSKgel Amide-80 column and a mobile phase compose...
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In proteomics, nanoflow multidimensional chromatography is now the gold standard for the separation of complex mixtures of peptides as generated by in-solution digestion of whole-cell lysates. Ideally, the different stationary phases used in multidimensional chromatography should provide orthogonal separation characteristics. For this reason, the combination of strong cation exchange chromatogr...
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In this paper, chromatographic analysis of active substance olopatadine hydrochloride, which is used in eye drops as antihistaminic agent, and its impurity E isomer by hydrophilic interaction liquid chromatography (HILIC) and application of design of experiments (DoE) methodology are presented. In addition, benzalkonium chloride is very often used as a preservative in eye drops. Therefore, the ...
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Many proteins in cells and biological Suids are glycosylated, and these glycoproteins are present in animals, plants, microorganisms and viruses. The most commonly occurring monosaccharides found in oligosaccharide attachments to mammalian proteins are D-mannose (Man), D-galactose (Gal), D-glucose (Glu), L-fucose (Fuc), N-acetylglucosamine (GlcNAc), N-acetylgalactosamine (GalNAc), and Nacetylne...
متن کاملHydrophilic interaction chromatography
In the early 1990s, hydrophilic interaction (liquid) chromatography (HILIC) emerged as a new separation method. HILIC is becoming increasingly popular for the separation of polar compounds in aqueous-organic mobile phases rich in organic solvents (usually acetonitrile). It has become important for the separation of pharmaceuticals, neurotransmitters, nucleosides, nucleotides and other compounds...
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ژورنال
عنوان ژورنال: Molecules
سال: 2014
ISSN: 1420-3049
DOI: 10.3390/molecules19079070